SBC8803: Zebrafish Gut-Brain Multi-Omics Data for Anxiolytic Psychobiotic Study

Multi-omics data from a 4-week study of adult male zebrafish fed the heat-killed paraprobiotic Lactobacillus brevis SBC8803. The dataset includes brain RNA sequencing and 16S rRNA amplicon sequencing of intestinal contents, integrated via GSVA and DIABLO-based analysis. Authored by Azusa Kubota and shared under CC-BY-4.0 on figshare in May 2026.

Use Cases

• Identify gut microbiome functional shifts linked to anxiolytic effects based on predicted metabolic pathways like riboflavin and tryptophan metabolism.
• Analyze coordinated changes between brain signaling and gut microbial pathways based on the integrated DIABLO and GSVA results.
• Investigate innate gut-brain coordination independent of treatment based on residual correlation analysis findings, such as coupling between brain arachidonic acid and gut histidine metabolism.
• Validate serotonin receptor, CREB, and oxytocin signaling pathways in the brain as potential mediators of reduced anxiety-like behavior.

Strengths

• Integrates two omics layers: brain transcriptomics and gut microbiome 16S rRNA sequencing.
• Includes results from specific analytical methods: Gene Set Variation Analysis (GSVA) and DIABLO-based data integration.
• Documents a clear 4-week experimental intervention with a defined paraprobiotic (heat-killed Lactobacillus brevis SBC8803).
• Links behavioral outcomes (novel tank test) to molecular mechanisms.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is contained within a 5.4 MB DOCX file, which may require extraction and formatting for analysis.

Provenance

Source

figshare, author Azusa Kubota.

Collection Method

Experimental study: adult male AB-strain zebrafish fed a diet containing heat-killed SBC8803 for 4 weeks, followed by behavioral testing, brain RNA-seq, and gut 16S rRNA sequencing.

Time Range

The experimental period lasted 4 weeks; specific calendar dates are not provided.

Freshness

Last updated 2026-05-21 05:35:20; freshness should be verified.