Cryptosporidium Parvum Rhomboid Protease Phylogeny and Localization

Ilaria Vanni's 2026 research article analyzes the rhomboid protease repertoire in the Cryptosporidium parvum parasite. The study details the phylogenetic origin, structural motifs, and subcellular localization of three rhomboids (CpRom1, CpRom2, CpRom3) in sporozoite cells. It compares these proteases across Cryptosporidium species and lists 10 candidate substrate proteins.

Use Cases

• Train models to classify rhomboid protease types (e.g., PARL-like vs. mixed secretases) based on phylogenetic and structural motif data.
• Analyze the spatial co-distribution of rhomboid proteases (CpRom1, CpRom2, CpRom3) and their 10 candidate substrate proteins for interaction prediction.
• Compare chromosomal synteny and gene excision events for PARL-like rhomboids across intestinal and gastric Cryptosporidium species.
• Cluster rhomboid proteases from C. parvum, Toxoplasma gondii, and Plasmodium falciparum based on catalytic site similarity data.
• Map subcellular localization patterns (apical complex, posterior pole, entire surface) for the three rhomboids in invasive sporozoites.

Strengths

• Identifies and characterizes three specific rhomboid proteases (CpRom1, CpRom2, CpRom3) in C. parvum.
• Provides phylogenetic analysis comparing rhomboids across multiple Cryptosporidium species and other apicomplexans.
• Lists 10 candidate substrate proteins based on similarity to proven substrates and subcellular co-presence.

Limitations

• Dataset is a single 86.0 KB PDF document, not a structured data table, limiting direct computational analysis.
• No raw experimental data (e.g., sequence alignments, imaging raw files) is provided, only analysis and conclusions.
• Temporal coverage and sample size for the underlying experimental work are not specified in the description.

Provenance

Source

Ilaria Vanni via figshare.

Collection Method

Bioinformatics and experimental analysis of phylogenetic origin, structural motifs, and cellular localization.

Time Range

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Freshness

Last updated April 2026, indicating recent analysis.

Geography

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