Kentucky Bluegrass Transcriptomics Data for Wax and Osmotic Stress Traits

228,310 unigenes were identified from a de novo transcriptome assembly of three Kentucky bluegrass cultivars with contrasting wax-associated epidermal structures. The dataset, authored by Xue You and last updated in May 2026, highlights a candidate 1R-MYB gene (PpMYBS3) linked to wax density and stress inducibility. It includes results from comparative phenotyping, differential expression analysis, and transgenic validation experiments.

Use Cases

• Identifying differentially expressed genes associated with wax crystal density based on pairwise comparisons of three cultivars.
• Analyzing enrichment of hormone signal transduction pathways in stress responses based on the transcriptomics data.
• Validating candidate gene function (e.g., PpMYBS3) in regulating wax biosynthesis genes and root architecture.
• Correlating phenotypic traits like stomatal aperture and ROS accumulation with gene expression patterns under osmotic stress.

Strengths

• Transcriptome assembly has a BUSCO completeness score of 76.8%.
• Differential expression analysis identified between 7,257 and 29,713 genes across cultivar comparisons.
• Includes validation data from stable overexpression lines showing phenotypic and gene expression changes.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (212.1 KB), indicating limited scope, likely containing summary or key results rather than raw sequencing data.
• Direct quantification of wax composition and cuticular water-loss assays are noted as missing, requiring further experiments.

Provenance

Source

figshare

Collection Method

De novo transcriptome assembly from pooled reads of nine libraries, combined with comparative phenotyping and transgenic validation.

Time Range

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Freshness

Last updated 2026-05-20 04:34:27; freshness should be verified.

Geography

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