Data Sheet 5_Transcriptomic profiling and targeted validation reveal molecular mechanisms

Data Sheet 5 contains transcriptomic and molecular validation data from a study investigating normobaric and hyperbaric oxygen therapy for high-altitude cerebral injury. The dataset, authored by Xiaojie Hu and last updated in April 2026, includes results from RNA sequencing, histopathology, and assays for oxidative stress and inflammatory markers in a mouse model. The file is a 54.7 KB PDF published under a CC-BY-4.0 license on figshare.

Use Cases

• Analyze transcriptomic pathway enrichment (e.g., NF-κB, PI3K–AKT, IL-17) based on KEGG analysis results mentioned in the description
• Compare molecular effects of different oxygen therapies (NBO vs. HBO) based on the described experimental groups and validation results
• Investigate correlations between oxidative stress markers (SOD, MDA, GSH, NO) and neuroinflammatory cytokine levels (IL-1β, IL-6, TNF-α) described in the study
• Validate gene expression patterns related to TLR4–NF-κB and PI3K–AKT/Nrf2 signaling axes described as key mechanistic pathways

Strengths

• Includes multi-modal validation data from RNA-seq, Western blot, immunofluorescence, and ELISA assays as described
• Experimental design includes four distinct treatment groups (Control, HH, NBO, HBO) for comparative analysis
• Published under a permissive CC-BY-4.0 license allowing reuse and redistribution

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The primary data is embedded within a 54.7 KB PDF, which may require extraction and parsing

Provenance

Source

Xiaojie Hu via figshare

Collection Method

Data generated from a controlled laboratory study using a mouse model in a hypobaric hypoxia chamber, with subsequent RNA sequencing and molecular assays.

Time Range

The study period is not specified, but the dataset metadata was updated in April 2026.

Freshness

Last updated 2026-04 13 05:49:14; freshness should be verified

Geography

The geographic origin of the study or data collection is not specified.