SLST-Seq: Strain-Level Cutibacterium acnes Profiles from Skin Samples

A dataset presenting results from the SLST-Seq method for strain-level profiling of Cutibacterium acnes from skin-strip samples. The method was validated using single-isolate controls, genomic DNA mixtures, and dilution series, demonstrating high specificity and reproducibility. The data was generated by Elody Orcel and last updated on 2026-05-20.

Use Cases

• Analyzing inter-individual variability in C. acnes communities based on SLST profiles from healthy volunteers.
• Validating quantitative accuracy of strain profiling methods based on target-to-background DNA ratio tests described.
• Studying conservation of donor-specific community structures between different skin sites like face and back.

Strengths

• Method validation includes single-isolate controls, defined genomic DNA mixtures, and spike-in dilution series.
• Demonstrated high specificity, quantitative accuracy, and strong run-to-run reproducibility per the description.
• Data is shared under a permissive CC-BY-4.0 license.

Limitations

• Dataset is very small at 9.5 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare, author Elody Orcel

Collection Method

Culture-independent SLST-Seq approach using LUMI-Seq® synthetic long-read sequencing on skin-strip samples.

Freshness

Last updated 2026-05-20 10:46:13; freshness should be verified.