Rhomboid Protease Analysis in Cryptosporidium Parvum Parasite

Cryptosporidium parvum rhomboid proteases are analyzed for phylogeny, structure, and cellular localization. The study identifies three rhomboids (CpRom1, CpRom2, CpRom3) and 10 candidate substrate proteins. Author Ilaria Vanni published this analysis in April 2026.

Use Cases

• Compare phylogenetic origins of CpRom1, CpRom2, and CpRom3 with orthologs from Toxoplasma gondii and Plasmodium falciparum.
• Analyze structural motifs and catalytic site similarities to classify rhomboids as mixed secretases.
• Map subcellular localization patterns of the three rhomboids within sporozoite cells.
• Investigate the genomic absence of PARL-like rhomboids in intestinal Cryptosporidium species versus gastric species.
• Identify candidate substrate membrane proteins based on similarity to known apicomplexan rhomboid substrates.

Strengths

• Provides detailed analysis of three specific rhomboid proteases (CpRom1, CpRom2, CpRom3).
• Includes comparative phylogenetic analysis across multiple Cryptosporidium species and other apicomplexans.
• Maps subcellular localization for each rhomboid within the invasive sporozoite stage.

Limitations

• Dataset is a single 102.5 KB PDF document, not a structured data table for computational analysis.
• No raw experimental data or quantitative measurements are provided in a machine-readable format.
• Analysis is specific to Cryptosporidium parvum, limiting direct applicability to other parasite systems.

Provenance

Source

figshare, authored by Ilaria Vanni.

Collection Method

Bioinformatic and phylogenetic analysis combined with cellular localization study.

Time Range

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Freshness

Last updated April 2026.

Geography

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