TP53 H179 Mutation Analysis in NSCLC: Molecular Dynamics and Genomic Characterization

A 2026 study by Ankur Datta analyzes mutational profiles from 1,160 individuals (616 LUAD and 544 LUSC) sourced from TCGA. The research focuses on specific TP53 H179 zinc-binding motif variants in Non-Small Cell Lung Cancer, using molecular dynamics simulations to assess structural and functional impacts. The document details mutation frequencies, conformational signatures, and binding affinity changes for five identified amino acid substitutions.

Use Cases

• Study mutation-induced structural plasticity in p53 based on analysis of L1 and L2 loop perturbations.
• Compare residue-level interaction patterns between wildtype and mutant proteins using contact map data.
• Analyze the relationship between specific nucleotide substitutions (e.g., C > A) and protein stability in cancer cohorts.
• Evaluate the pathogenic potential of zinc-binding motif mutations based on conformational trajectory analysis.
• Investigate differential tumor-suppressive function modulation based on specific amino acid substitutions at the H179 locus.

Strengths

• Analysis covers a substantial cohort of 1,160 NSCLC patients from TCGA.
• Provides specific mutation frequencies: TP53 mutations in 50% of LUAD and 81% of LUSC cases.
• Focuses on a structurally important but underexplored site (His179) within the p53 zinc-binding motif.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The primary data file is a DOCX document, which may require extraction or conversion for computational analysis.

Provenance

Source

figshare, author Ankur Datta.

Collection Method

TCGA mutational profiles were evaluated, with structural analysis performed via molecular dynamics simulations.

Time Range

Temporal coverage of the underlying TCGA data is not specified.

Freshness

Last updated 2026-04-10 05:59:23; freshness should be verified.

Geography

Spatial coverage is not specified.