SLST-Seq: Strain-Level Typing of Cutibacterium acnes from Skin Samples

SLST-Seq is a dataset presenting a culture-independent, single-locus sequence typing method for phylotyping Cutibacterium acnes from skin samples. The method was validated using single-isolate controls, genomic DNA mixtures, and spike-in dilution series, demonstrating high specificity and quantitative accuracy. The dataset, authored by Elody Orcel and last updated in May 2026, contains results from applying SLST-Seq to skin-strip samples from healthy volunteers.

Use Cases

• Validate strain-level profiling methods based on the described single-locus sequence typing (SLST) marker.
• Study inter-individual variability in skin microbiome composition based on the SLST profiles from healthy volunteers.
• Benchmark quantitative accuracy for microbiome analysis based on the spike-in dilution series validation data.
• Investigate site-specific conservation of microbial communities based on the comparison of face and back skin samples.

Strengths

• Method validated with specific controls including single-isolate controls and defined genomic DNA mixtures.
• Demonstrated strong run-to-run reproducibility as stated in the result description.
• Dataset is openly licensed under CC-BY-4.0.

Limitations

• Dataset is very small at 10.2 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare, author Elody Orcel

Collection Method

Culture-independent SLST-Seq method combining unique molecular identifier barcoding with de novo assembly.

Freshness

Last updated 2026-05-20 10:46:10; freshness should be verified.