Rat Hippocampus Gene Expression After CDNF Overexpression via RT-qPCR

Absolute and relative RT-qPCR analysis measured mRNA levels of 11 genes in the dorsal hippocampus of adult male Wistar rats. The dataset, authored by Anton Tsybko and last updated in May 2026, compares gene expression four weeks after stereotaxic injection of an AAV-Syn-CDNF-EGFP vector against a control group. The 24.7 KB ZIP file contains results for genes including Cdnf, Creb1, Perk, Atf6, Ire1, Grp78, spliced and unspliced Xbp1, and Bdnf exons I, II, IV, VI.

Use Cases

• Compare gene expression levels between CDNF-overexpressing and control rat hippocampi based on the described RT-qPCR analysis.
• Analyze the effect of CDNF on specific Bdnf exon expression based on the relative quantification data for exons I, II, IV, and VI.
• Investigate endoplasmic reticulum stress marker genes (Perk, Atf6, Ire1, Grp78, Xbp1 splicing) in a neurotrophic factor overexpression model.
• Validate absolute quantification methods for neurobiology research based on the copy number data normalized to Polr2a.

Strengths

• Data includes absolute quantification for 6 genes (Cdnf, Creb1, Perk, Atf6, Ire1, Grp78) and relative quantification for Bdnf exons and Xbp1 splicing.
• Experimental design is clearly described, specifying animal model (adult male Wistar rats), vector (AAV-Syn-CDNF-EGFP), and a 4-week post-injection time point.
• Dataset is openly licensed under CC-BY-4.0, permitting reuse with attribution.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• The dataset's 24.7 KB size suggests a limited scope, likely containing summary-level results rather than raw sequencing reads.
• Data may reflect bias inherent to the specific experimental model (single rat strain, single time point, male-only subjects).

Provenance

Source

Anton Tsybko via figshare

Collection Method

Total RNA was extracted from dissected hippocampi, reverse-transcribed, and analyzed by real-time quantitative PCR.

Freshness

Last updated 2026-05-24 10:45:49