E. coli qPCR Method Performance Data for Great Lakes Recreational Waters

Results from an extensive survey document the performance of a modified qPCR method for analyzing E. coli fecal indicator bacteria in recreational waters. The dataset, provided by the U.S. Environmental Protection Agency, supports the method's adoption into a Standard Operating Procedure for Michigan beach water testing. It includes data to determine when and where DNA recovery losses may occur with and without the modification.

Use Cases

• Evaluating a modified qPCR method's performance based on the reported results and survey data
• Determining conditions for DNA recovery losses in beach water testing based on the extensive survey
• Supporting the adoption of a Standard Operating Procedure for E. coli analysis in Michigan
• Comparing DNA recovery with and without the method modification for Great Lakes region waters

Strengths

• Data from an extensive survey mentioned in the description
• Results have led to adoption into a Standard Operating Procedure for Michigan labs
• Provenance from the U.S. Environmental Protection Agency

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count and dataset size are unknown, which may limit suitability assessment

Provenance

Source

U.S. Environmental Protection Agency

Collection Method

Results from qPCR method testing and an extensive survey, as reported by EPA/ORD

Time Range

null

Freshness

Last updated 2026-03-22 11:49:52.725401; freshness should be verified

Geography

Great Lakes region, with specific mention of Michigan recreational beaches