Microscope Autofocus Images and Code for Automating Blood Sample Imaging

Example images and analysis code support the development of a smart stack procedure for closed-loop autofocus on a motorised microscope. The data includes examples of image processing, tiling, and analysis to find the focal plane. It was created by Joe Knapper at the University of Bath.

Use Cases

• Developing autofocus algorithms based on the described smart stack procedure.
• Testing image processing and tiling methods for large microscope scans.
• Analyzing the distribution of sharpest images within focus stacks across multiple scans.

Strengths

• Includes example images and analysis code, providing a practical starting point.
• Focuses on a specific, documented application: automating blood sample imaging on the OpenFlexure Microscope.

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

University of Bath

Collection Method

Created to support the development and publication of a smart stack autofocus procedure.