Lacticaseibacillus rhamnosus HN001 In Vitro Gut-Brain Barrier Integrity Measurements

An in vitro dataset from a two-stage gut-brain axis model investigating the probiotic Lacticaseibacillus rhamnosus HN001. The data, uploaded by Dulantha Ulluwishewa on 2026-04 14, includes measurements of transepithelial electrical resistance and transcriptional modulation of tight-junction genes in Caco-2 and hCMEC/D3 cell monolayers under various conditions.

Use Cases

• Modeling probiotic effects on intestinal barrier integrity based on transepithelial electrical resistance data.
• Analyzing tight-junction gene expression modulation (e.g., OCLN, CLDN2) in response to bacterial treatment.
• Investigating cross-barrier protection mechanisms using conditioned medium transfer data between intestinal and brain endothelial models.
• Assessing cytokine-independent barrier regulation based on IL-1β-induced disruption mitigation data.

Strengths

• Data is from a controlled two-stage in vitro model, allowing mechanistic investigation.
• Includes measurements under both aerobic and apical-anaerobic conditions, providing environmental context.
• Released under a permissive CC-BY-4.0 license for reuse.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data is from a specific in vitro model; direct applicability to in vivo systems is limited.

Provenance

Source

figshare, authored by Dulantha Ulluwishewa.

Collection Method

Generated from a laboratory in vitro co-culture model using differentiated Caco-2 and hCMEC/D3 cell monolayers.

Time Range

Experimental timeframe not specified.

Freshness

Last updated 2026-04-14 21:12:50.

Geography

Laboratory study; geographic origin not specified.