Live-Cell STED Microscopy Images at 50 nm Resolution with Cell Proliferation Data

Stimulated emission depletion microscopy images of living human cells. The dataset includes long-term 20-hour studies on cell proliferation and short-term observations of cytosolic calcium levels. It was created by Mol, F.N. and was last updated on 2026-05-25.

Use Cases

• Train models for super-resolution image segmentation based on images of nuclear pore complexes, Golgi, mitochondria, and actin.
• Analyze cell proliferation and survival rates based on wide-field epifluorescence time-lapse data.
• Study cellular stress responses based on cytosolic calcium level measurements during imaging.

Strengths

• Achieves 50 nm resolution imaging.
• Includes long-term 20-hour studies on cell proliferation.
• Covers multiple human cell lines (U2OS, HeLa, RPE-1) and subcellular structures.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

Mol, F.N.

Collection Method

Stimulated emission depletion (STED) microscopy and wide-field epifluorescence time-lapse imaging.

Freshness

Last updated 2026-05-25 06:10:23; freshness should be verified.