DcuSEnvZ_Barcode_Counts: Mutagenized Sensor Kinase Signaling Phenotypes

1,173 mutations across chimeric DcuS/EnvZ sensor histidine kinases were screened for signaling outputs in response to ligands fumarate and aspartate. Lucas Lippert deposited these barcode-count files for analysis on GitHub, with raw sequencing data available on the NIH Sequence Read Archive. The dataset was last updated on May 21, 2026.

Use Cases

• Analyze sequence-function relationships based on mutagenized sensory and transmembrane domains.
• Identify positions altering ligand selectivity based on aspartate responsiveness.
• Screen mutagenized libraries of natural sensor histidine kinases for biosensor design.
• Quantify signaling outputs based on standardized fluorescence readouts from a synthetic system.

Strengths

• Quantifies effects of 1,173 mutations on signaling outputs.
• Links to a GitHub repository with R analysis scripts for reproducibility.
• Raw sequencing data is available on the NIH Sequence Read Archive under BioProject PRJNA1468850.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect experimental bias inherent to the specific synthetic system.

Provenance

Source

figshare

Collection Method

High-throughput screening of a library of chimeric DcuS/EnvZ sensor histidine kinases.

Freshness

Last updated 2026-05-21 21:24:08