JHU_Msex_v1.0: De Novo Genome Assembly of the Tobacco Hornworm Moth

Johns Hopkins University presents JHU_Msex_v1.0, a new reference genome for the insect Manduca sexta (Tobacco Hornworm moth). The 470 Mb assembly is approximately 25 times more continuous than its predecessor, with a scaffold N50 greater than 14 Mb. It includes annotations for 25,256 genes, derived from existing annotations supplemented with RNA-based data.

Use Cases

• Comparative genomics analysis based on the annotated 25,256 genes.
• Studying genome structure and evolution using the high-continuity assembly with scaffold N50 >14 Mb.
• Functional annotation and gene discovery leveraging the RNA-based supporting data.
• Serving as a reference for population genetics or resequencing studies of Manduca sexta.

Strengths

• High assembly continuity, being ~25x more continuous than the original with a scaffold N50 >14 Mb.
• Comprehensive gene annotation, totaling 25,256 genes.
• Application of modern de novo assembly technologies to improve accuracy and completeness.

Limitations

• Key metadata such as specific file formats, row/record counts, and exact update dates are not provided across sources.
• The dataset's availability and access methods beyond being 'accessible in annotated form for public use' are unspecified.

Provenance

Source

Johns Hopkins University, authored by Ariel Gershman.

Collection Method

De novo genome assembly using a combination of modern technologies, with annotations lifted from existing sources and supplemented with RNA-based data.