Tumor Spheroid Deformation Field Imaging Over Two Hours

Two hours of continuous confocal microscopy imaging captures deformation fields caused by tumor spheroid contractility in a 3D collagen ECM. The dataset includes tracer particles and photocrosslinked ECM patches, with frames recorded every 15 minutes. It was created by Bo Sun and shared under a CC BY 4.0 license.

Use Cases

• Analyze deformation field patterns from tracer particle displacements over the 2-hour imaging sequence.
• Measure contractility dynamics of the tumor spheroid by tracking changes in the ECM structure between frames.
• Validate computational models of mechanical stress in 3D collagen environments using the observed deformation data.

Strengths

• Imaging performed on a Leica TCS SPE confocal microscope with a 10x air, 0.40 NA objective, providing detailed spatial resolution.
• Continuous temporal coverage over 2 hours with a frame captured every 15 minutes.
• Dataset includes multiple experimental components: tracer particles, photocrosslinked ECM patches, and a tumor spheroid.

Limitations

• Small dataset size of 14.5 MB suggests limited spatial or temporal granularity.
• Unknown row and column structure complicates immediate quantitative analysis.
• Experimental setup is specific to a single tumor spheroid and collagen ECM type, limiting generalizability.

Provenance

Source

Bo Sun via figshare.

Collection Method

Continuous confocal microscopy imaging of tracer particles in a 3D type I collagen ECM with a tumor spheroid.

Time Range

Imaging duration of 2 hours.

Freshness

Last updated March 13, 2026.

Geography

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