Aromatic Cation Probes for Protein Labeling and Live Cell Imaging

1.9 MB of experimental data on aromatic cation salts designed for photoinduced protein ligation. The dataset, shared by Pranab C. Saha on figshare, includes structure–reactivity relationships for probes enabling labeling with green light. It contains results from mass spectrometry-based proteomic analysis showing distinct protein enrichment from mitochondria and the endoplasmic reticulum.

Use Cases

• Developing new photoinduced protein labeling strategies based on structure–reactivity relationships of aromatic cations.
• Training models to predict probe reactivity or photophysical properties based on extended conjugation and donor–acceptor relationships.
• Analyzing proteomic enrichment patterns for probes targeting specific organelles like mitochondria and the endoplasmic reticulum.
• Designing wash-free, live cell imaging protocols using probes with state-dependent photophysical properties.

Strengths

• Data includes mass spectrometry results showing highly distinct proteomic subsections with less than 10% protein enrichment overlap.
• Probes were validated for both photoinduced protein labeling and wash-free, live cell imaging, demonstrating dual functionality.
• File is provided in the structured XLSX format, which is 1.9 MB in size.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Systematic study of structure–reactivity relationships of donor–acceptor pyridinium salts, with validation via mass spectrometry and live cell imaging.

Freshness

Last updated 2026-04-15 20:30:48; freshness should be verified.