Detection of antimicrobial resistance genes in beta-lactamase- and carbapenemase-producing

Seventy Klebsiella pneumoniae isolates from rectal swabs were collected at an intensive care unit in Rio de Janeiro, Brazil, from March 2013 to March 2014. The dataset, described in a paper by Claudia Flores, includes antimicrobial susceptibility results and the presence of nine specific resistance genes. It reveals high resistance rates to several antibiotics and a high degree of genetic diversity among the isolates.

Use Cases

• Modeling the co-occurrence of multiple resistance genes based on the 29 observed resistance gene profiles.
• Analyzing genetic diversity and strain relatedness based on the 68 distinct ERIC-PCR profiles.
• Investigating correlations between phenotypic resistance rates (e.g., to cefepime, ertapenem) and the presence of specific beta-lactamase genes.
• Studying the geographic and temporal spread of specific resistance mechanisms like blaKPC and blaCTX-M-15 in a hospital setting.

Strengths

• Includes 70 distinct bacterial isolates, providing a substantive sample for analysis.
• Reports specific resistance rates for six antibiotics, such as 96% to ceftazidime and 61% to ertapenem.
• Identifies prevalence for six different resistance genes, with blaSHV at 69% and blaKPC at 57%.
• Documents high genetic diversity with 68 unique ERIC-PCR profiles among the isolates.

Limitations

• Row count for the underlying data table is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data may reflect geographic and temporal bias inherent to a single ICU study in Brazil from 2013-2014.

Provenance

Source

Claudia Flores (via paperswithcode)

Collection Method

Seventy isolates obtained from rectal swab surveillance cultures; antimicrobial susceptibility tested by VITEK 2 System; resistance genes detected by PCR; genetic diversity assessed by ERIC-PCR.

Time Range

March 2013 to March 2014

Geography

Intensive care unit in Rio de Janeiro, Brazil