Hemopathogen Detection Data from Goats, Sheep, Donkeys, and Biting Keds

Data files from Rotor Gene, Quant Studio, and micPCR HRM instruments for detecting pathogens in livestock and associated biting keds. The dataset includes HRM data for Anaplasma spp. and Ehrlichia sp., accessible via specific software platforms, with a no-template negative control (NTC) included. It was contributed by author Daniel M Mwaki and is available under an Open Access (green) license.

Use Cases

• Validate PCR-based diagnostic assays for Anaplasma spp. based on the provided HRM data files.
• Compare detection sensitivity across different PCR platforms (Rotor Gene, Quant Studio, micPCR) using the instrument data.
• Study pathogen prevalence in livestock (goats, sheep, donkeys) and their ectoparasites (biting keds) using the detection results.
• Develop bioinformatics pipelines for analyzing High-Resolution Melting (HRM) curve data from the described pathogens.

Strengths

• Includes data from three established PCR instrument platforms (Rotor Gene, Quant Studio, micPCR), suggesting methodological cross-validation.
• Specifically mentions HRM data for Anaplasma spp. and Ehrlichia sp., providing targeted pathogen information.
• Contains a defined negative control (NTC), which is essential for assay quality control.

Limitations

• Row count, file size, and specific column definitions are unknown, limiting suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

Daniel M Mwaki

Collection Method

Data collected via Rotor Gene, Quant Studio, and micPCR HRM instruments for pathogen detection.