Detection of Leifsonia xyli in Presprouted Sugarcane Seedlings

52 presprouted sugarcane seedlings aged 45 days were tested for the bacterial pathogen Leifsonia xyli subsp. xyli (Lxx). This diagnostic assay data was developed by Oliveira Danielle De to enable early detection of ratoon stunt disease in planting materials. The study evaluated four primer pairs under original and modified PCR conditions to identify the most accurate detection method.

Use Cases

• Compare the efficacy of different PCR primer pairs for detecting Lxx based on the described assay conditions.
• Validate early disease detection protocols for presprouted seedcane (MPB) based on the 45- and 60-day-old plant samples mentioned.
• Assess diagnostic accuracy for various pathogen titers (e.g., 10^6 to 10^9 CFU/mL) as referenced in the description.

Strengths

• Includes results from testing 52 individual MPB samples aged 45 days.
• Compares the diagnostic performance of four specific primer pairs (Cxx ITSf#5/Cxx ITSr#5, Cxx1/Cxx2, C2F/C2R, LX23SF/LX23SR).
• Evaluates detection across a range of bacterial concentrations (10^6 to 10^9 CFU/mL).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count for the full dataset is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

paperswithcode

Collection Method

Experimental assay data from a study developing a PCR-based diagnostic test.

Geography

Likely Brazil, inferred from the description's focus on the Brazilian sugarcane industry.