L-Ascorbic Acid Concentrations in Raw and Processed Milk Samples

A method for determining L-ascorbic acid in milk using UHPLC-MS/MS was validated for linearity, accuracy, and precision. The study reports a limit of detection of 1.5 µg L⁻¹ and a limit of quantification of 5.0 µg L⁻¹. The method was applied to milk samples by author Caroline Zappielo.

Use Cases

• Validate a UHPLC-MS/MS method for vitamin C analysis based on the described protocol.
• Compare L-ascorbic acid levels between raw and processed milk based on the sample types mentioned.
• Assess method performance metrics like recovery and precision based on the reported validation results.
• Benchmark new analytical techniques against the reported low detection limits of 1.5 µg L⁻¹.

Strengths

• Method validation includes specific metrics: linearity (r² > 0.9915), LOD (1.5 µg L⁻¹), LOQ (5.0 µg L⁻¹).
• Recovery rates for spiked samples were higher than 90%, and precision (RSD) was ≤ 6.8%.
• The description details the use of low-toxicity solvents and stabilizing agents like EDTA and formic acid.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

Caroline Zappielo via paperswithcode

Collection Method

Analytical chemistry using ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS).