Alpha-Synuclein Monomer and Fibril Interactomes from Mouse Brain Lysate

Protein interaction data for alpha-synuclein monomers and fibrils, generated via micromap photoproximity labeling in mouse brain lysate. Marshall G. Lougee published the dataset in April 2026. The data includes validation against prior proximity labeling sets and further investigation examples.

Use Cases

• Compare monomer and fibril interactomes to identify proteins associated with toxic gain-of-function in synucleinopathies.
• Validate proximity labeling results using included Western blotting and affinity pulldown data.
• Analyze interactome differences between specific regions of the alpha-synuclein protein using the minimally perturbing Ir catalyst.
• Investigate protein interactions in primary neuronal contexts using the provided micromap protocol.

Strengths

• Interactomes generated for both disease-relevant alpha-synuclein fibrils and monomers for direct comparison.
• Method uses a 1 kDa Ir catalyst with a narrow labeling radius for precise regional interaction mapping.
• Dataset validated against previous proximity labeling data sets.

Limitations

• Small dataset size of 1.3 MB suggests limited raw data volume.
• Data derived solely from mouse brain lysate, limiting direct human biological relevance.
• Unknown row count and specific column structure prevent assessment of sample size.

Provenance

Source

figshare, authored by Marshall G. Lougee.

Collection Method

Generated via micromap (μMap) photoproximity labeling on synthesized alpha-synuclein variants in mouse brain lysate.

Time Range

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Freshness

Last updated in April 2026.

Geography

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