Flavonoid Concentrations in Vochysia Divergens Under Water Stress and Fungal Inoculation

The article describes the development and validation of an HPLC-DAD analytical method to quantify three 5-methoxyflavones in methanolic extracts of Vochysia divergens Pohl. The method was applied to plants grown in a greenhouse, associated with endophytic fungi Zopfiella tetraspora or Melanconiella elegans, and subjected to water stress. Letícia Pereira Pimenta authored the research, which found that inoculation with Z. tetraspora decreased the concentration of one flavone by 2.69-fold compared to the control.

Use Cases

• Analyzing the effect of endophytic fungal inoculation on plant flavonoid production based on quantified concentrations of three specific flavones.
• Studying plant biochemical responses to water stress based on the described experimental conditions.
• Validating HPLC-DAD analytical methods for quantifying specific 5-methoxyflavones in plant extracts.
• Investigating the symbiosis between Vochysia divergens and fungal microorganisms in the Pantanal biome.

Strengths

• The analytical method was validated, suggesting a defined and reliable quantification process.
• The study quantifies three specific flavones (3',5-dimethoxy luteolin-7-O-β-glucopyranoside, 5-methoxy luteolin, and 3',5-dimethoxy luteolin).
• Experimental design includes controlled variables: greenhouse growth, specific fungal inoculations (Zopfiella tetraspora, Melanconiella elegans), and water stress.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect a specific experimental bias inherent to the single study described on paperswithcode.

Provenance

Source

Letícia Pereira Pimenta via paperswithcode.

Collection Method

Data likely originates from laboratory HPLC-DAD analysis of methanolic extracts from greenhouse-grown plants.

Geography

Plant species (Vochysia divergens) is associated with the Pantanal biome in Brazil.