Spodoptera frugiperda Molecular Identification Data for LAMP Assay

Juil Kim from the Institute of Crop Science developed a molecular tool for identifying the invasive fall armyworm, Spodoptera frugiperda. The method uses a species-specific sequence region identified from mitochondrial genome alignment and applies Loop-Mediated Isothermal Amplification (LAMP) assays. The LAMP assay can detect DNA concentrations as low as 10 pg and can be applied to field-collected larval and adult samples without standard DNA extraction.

Use Cases

• Field monitoring of Spodoptera frugiperda based on the described LAMP assay protocol
• Ecological studies of invasive pest spread based on species-specific genetic markers
• Validation of molecular diagnostic primers designed from mitochondrial tRNA regions
• Comparison of LAMP and conventional PCR methods for insect species identification
• Testing DNA release techniques from tissue samples without full DNA extraction

Strengths

• The LAMP assay has a defined minimum detectable DNA concentration of 10 pg
• Optimal incubation conditions are specified as 61°C for 90 minutes
• The method was validated on field-collected samples
• The assay can be used with a simplified DNA release technique, bypassing full extraction

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Data may reflect geographic bias inherent to paperswithcode, focusing on a specific research project

Provenance

Source

Institute of Crop Science

Collection Method

Molecular assay development using mitochondrial genome sequence alignment and LAMP primer design.

Geography

Field-collected samples; the pest is invasive in African and Asian countries.