21 DIA proteomics samples from mouse forelimb skeletal muscle at 7 ages from 3 to 26 months. Yu-Jie Zhang generated a spectral library using DDA and transcriptome data, analyzed with Spectronaut. The submission includes raw files and a processed protein expression matrix.
Use Cases
- Model age-related protein expression trajectories based on the seven distinct time points
- Identify biomarkers of muscle aging from the processed protein expression matrix
- Benchmark DIA proteomics analysis pipelines using the provided raw files and spectral library
- Study longitudinal proteomic changes in a specific tissue (forelimb skeletal muscle)
Strengths
- Includes samples from 7 distinct ages (3, 9, 12, 18, 21, 24, and 26 months) providing a longitudinal view
- Contains both raw DIA files and a processed protein expression matrix for flexibility
- Uses 3 biological replicates per age group for statistical robustness
Limitations
- Column-level documentation is absent; field semantics must be inferred after download
- Row count is unknown, which may limit suitability assessment
Provenance
- Source
- figshare
- Collection Method
- DIA quantitative proteomics with a spectral library generated from DDA, full-length transcriptome data, and the UniProt database.
- Time Range
- Ages 3 to 26 months
- Freshness
- Last updated 2026-05-02 09:23:09; freshness should be verified