Diel Mouse Gut Study: 16S Amplicon Data from Low-Fat and High-Fat Diet Experiments

16S V4-V5 region amplicon sequences from mouse gut samples. Mice were fed low-fat or high-fat diets, with samples taken from feces, cecum, and ileum over 24-hour and 48-hour periods. The data was sequenced by Sean M. Gibbons and includes FASTQ files and mapping metadata for demultiplexing.

Use Cases

• Analyzing diurnal variation in gut microbial composition based on time-series sampling over 24 and 48 hours.
• Comparing microbiome profiles between low-fat and high-fat diet conditions mentioned in the description.
• Investigating microbial differences across gut compartments (feces, cecum, ileum) described in the sampling protocol.
• Developing or testing bioinformatics pipelines for processing forward-read 16S amplicon data.

Strengths

• Includes data from two distinct time-course experiments (24-hour and 48-hour).
• Samples were taken from multiple gut sites (feces, cecum, ileum) and from replicate mice.
• Provides mapping files with sample metadata for demultiplexing the sequencing runs.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Sequencing was performed in the forward direction only, which may limit taxonomic resolution.

Provenance

Source

Sean M. Gibbons

Collection Method

16S rRNA gene amplicon sequencing (V4-V5 region) of mouse gut samples.

Time Range

Samples were collected over 24-hour and 48-hour periods.