Acholeplasma Genome Transfer Data via Yeast Cell Fusion

Daniel P. Nucifora's dataset documents the direct transfer of the 1.46 Mb genome from Acholeplasma laidlawii strain DN-E to yeast using cell fusion. It includes results from 20 screened yeast colonies, with 13 confirmed to contain the bacterial genome via multiplex PCR. The data was published on figshare in April 2026.

Use Cases

• Analyze the success rate of genome transfer by evaluating the 13 positive colonies out of 20 screened.
• Study the role of the recA gene complementation in enabling genomic integration of the yeast vector.
• Validate multiplex PCR protocols for confirming the presence of the Acholeplasma genome in yeast hosts.
• Investigate the cell fusion method as a technique for cloning entire bacterial genomes without prior toxic gene deletion.

Strengths

• Documents the first demonstration of cloning entire Acholeplasma laidlawii genomes via direct cell fusion.
• Includes experimental validation on 20 yeast colonies with specific PCR results.

Limitations

• Dataset is very small at 92.0 KB, indicating limited raw data or supplementary files.
• Experimental scope is narrow, focusing on a single bacterial strain and method.

Provenance

Source

figshare, authored by Daniel P. Nucifora.

Collection Method

Experimental data from genome transfer via cell fusion and subsequent multiplex PCR screening.

Time Range

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Freshness

Last updated April 2026.

Geography

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