Direct Infusion ADE-MS/MS Lipidomics Data for NIST SRM 1950 Plasma

A 2026 dataset by Yu Zhang on figshare presents lipidomic data generated using a high-throughput acoustic droplet ejection mass spectrometry (ADE-MS/MS) workflow. The data includes quantification of 731 lipid species from NIST SRM 1950 plasma, with over 1000 species across 14 subclasses quantified in the method. The workflow achieved a total run time of approximately five minutes per sample in a 384-well format.

Use Cases

• Benchmarking new high-throughput lipidomics workflows based on the reported 5-minute-per-sample runtime and 384-well format.
• Analyzing lipid species abundance and dysregulation based on the quantified 731 lipid species from a standardized reference material.
• Validating lipid identification and quantification methods based on cross-platform comparison results with HILIC-MS/MS.
• Studying lipidomic changes associated with body mass index based on the 656 differential lipid features identified in the study.

Strengths

• Quantifies 731 lipid species from a standardized reference material (NIST SRM 1950 plasma).
• Method demonstrated strong analytical performance in linearity, precision, reproducibility, and recovery.
• Cross-platform validation showed strong agreement (R² > 0.80 for most subclasses) with an established HILIC-MS/MS method.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (118.6 KB), indicating it likely contains summary or processed results rather than raw spectra.

Provenance

Source

figshare, author Yu Zhang.

Collection Method

Data generated via an acoustic droplet ejection mass spectrometry (ADE-MS/MS) workflow with parallel reaction monitoring on a ZenoTOF MS platform.

Freshness

Last updated 2026-03-25 17:34:13; freshness should be verified.