Lysine Fumarylation Sites and Enzymatic Pathway in Escherichia coli

Chen Chen discovered lysine fumarylation, a bacterial post-translational modification. The dataset likely contains 857 endogenous fumarylation sites identified in Escherichia coli using mass spectrometry. The work was published on figshare in April 2026.

Use Cases

• Identify fumarylation sites based on the +98.0002 Da mass shift
• Analyze enzymatic regulation pathways based on the SucC–SucD, SpeG, and CobB enzymes
• Investigate stress response gene regulation based on integrated transcriptomic and proteomic analyses

Strengths

• 857 endogenous fumarylation sites were mapped
• The modification was confirmed through chromatographic retention and MS/MS fragmentation comparisons
• An enzymatic pathway (SucC–SucD, SpeG, CobB) regulating the modification was elucidated

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The dataset is 345.8 KB, indicating a limited scope

Provenance

Source

figshare

Collection Method

Identified via open-search mass spectrometry and enrichment with a pan-succinyl-lysine antibody.

Freshness

Last updated 2026-04-10 06:05:30