Flavophos Phosphonate Data from Burkholderia Heterologous Expression

Max A. Simon's research dataset on figshare describes the discovery of flavophos, a phosphonate antimicrobial compound. The data likely contains results from heterologous expression in Escherichia coli, spectroscopic characterization, chemical synthesis, and biochemical experiments on enzyme inhibition. The dataset was last updated on April 27, 2026.

Use Cases

• Study phosphonate biosynthetic gene clusters (BGCs) based on the described family of BGCs found in Burkholderia.
• Investigate novel antimicrobial compounds based on the heterologous expression and characterization of flavophos.
• Analyze enzyme inhibition mechanisms based on the described inhibition of lumazine synthase (LS) by 2,4-dioxopentylphosphonic acid.
• Characterize divergent enzyme catalysis based on the in vitro studies of a DUF849 family enzyme with homology to β-keto acid cleavage enzymes.

Strengths

• Dataset includes spectroscopic characterization and chemical synthesis data that assigned the structure of the compound as 2,4-dioxopentylphosphonic acid.
• Biochemical experiments revealed the compound's inhibition of lumazine synthase (LS), an essential enzyme in flavin biosynthesis.
• The dataset is associated with a specific research study, providing contextual relevance.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 1.6 MB file size suggests a relatively small dataset, potentially limiting scope.

Provenance

Source

figshare

Collection Method

Likely contains experimental data from heterologous expression, spectroscopy, synthesis, and biochemical assays as described in the study.

Freshness

Last updated 2026-04-27 04:46:06; freshness should be verified.