Immunopeptidome Ile/Leu Variant Data Using Hybrid ExCID Fragmentation

Data from a study using hybrid Electron-Activated Dissociation and Collision-Induced Dissociation (ExCID) mass spectrometry to distinguish isoleucine and leucine sequence variants in HLA-bound peptides. The dataset includes results from synthetic peptide panels and complex samples from two model cell lines, C1R-A*02:01 and Panc1. It was authored by Mohammadreza Dorvash and last updated in June 2026.

Use Cases

• Training models to differentiate isobaric amino acid variants based on diagnostic w- and d-type satellite ion patterns.
• Benchmarking fragmentation methods for nontryptic, short (8-12 mer) peptide identification in immunopeptidomics.
• Optimizing mass spectrometry parameters like kinetic energy and reaction time for hybrid ion activation workflows.

Strengths

• Includes data from a panel of seven synthetic Ile/Leu-substituted peptides used for method validation.
• Applies optimized methodology to complex samples from two distinct model cell lines.
• File size is 2.0 MB, indicating a focused and likely manageable dataset.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale training.
• Data may reflect methodological bias inherent to the specific SCIEX ZenoTOF 7600+ system and figshare platform.

Provenance

Source

figshare

Collection Method

Generated via tandem mass spectrometry (MS/MS) using hybrid EAD and CID fragmentation on a SCIEX ZenoTOF 7600+ system.

Freshness

Last updated 2026-06-02 15:09:58; freshness should be verified.