Stable Isotope Analysis of Crustacean Tissue Under Different Grinding Methods

An experimental dataset of ~50 mg freshwater crustacean muscle samples used to evaluate plastic contamination from mechanical grinding. The study analyzed carbon and nitrogen elemental composition and stable isotope values across four grinding methods: hand-ground and mill-ground for 1, 2, and 5 minutes. Results show no significant differences in δ¹⁵N or δ¹³C values among treatments, but an increase in %C with longer milling time.

Use Cases

• Assessing the impact of ball milling duration on carbon content based on the reported increase in %C with grinding time.
• Evaluating the suitability of mechanical grinding for small animal samples based on the analysis of ~50 mg crustacean muscle.
• Developing sample preparation protocols based on the graphical recommendation for minimum sample amount to avoid plastic contamination.
• Comparing isotopic consistency across preparation methods based on the reported δ¹⁵N and δ¹³C values for hand-ground versus mill-ground samples.

Strengths

• Experimental design includes four distinct grinding treatments (hand-ground, mill-ground for 1, 2, and 5 minutes).
• Dataset includes specific quantitative findings, such as an estimated plastic abrasion of 1.65 mg.
• Provides a concrete graphical recommendation for minimum sample amount to avoid contamination bias.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Dataset is small (16.0 KB), indicating a limited scope focused on a specific experimental result.

Provenance

Source

figshare

Collection Method

Experimental analysis of freshwater crustacean samples comparing hand-grinding and ball-milling homogenization methods.

Freshness

Last updated 2026-04-29 08:11:45; freshness should be verified.