Qiantang River Megalobrama Draft Genome Assembly and Reference Projection

kai liu published a draft genome assembly for a Qiantang River Megalobrama fish sample (SJF) on figshare in 2026. The dataset includes 100.14 Gb of filtered short-read sequencing data and the results of projecting the assembly onto two chromosome-level references. The final callable core sequence spans approximately 724.85–727.35 Mb.

Use Cases

• Evaluate assembly quality metrics based on BUSCO completeness scores and scaffold N50 statistics.
• Compare reference-assisted genome projection outcomes based on the use of NCBI versus Genome Warehouse (GWH) references.
• Analyze genome structure based on features like estimated size (~906.7–909.0 Mb), heterozygosity (1.18–1.20%), and repeat content (34.7%).
• Study callable genomic intervals based on the defined core and reference-sensitive shell regions.

Strengths

• Includes 100.14 Gb of filtered short-read sequencing data.
• Provides a callable core sequence of 724.85–727.35 Mb.
• Compares projection results using two distinct reference genomes (NCBI and GWH).
• Reports BUSCO completeness scores ranging from 95.5–97.9% after reference-assisted projection.

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

kai liu via figshare

Collection Method

Genome assembly from short-read sequencing of a Qiantang River Megalobrama sample (SJF), polished and projected onto two references.

Freshness

Last updated 2026-05-25 06:21:48; freshness should be verified.

Geography

Qiantang River