E11bio PRISM: Mouse Hippocampal Neurons with Multiplexed Protein Imaging

Mouse hippocampal CA3 neurons imaged using E11.bio's PRISM technology, which combines viral barcoding and expansion microscopy. The dataset includes light microscopy data, cell morphology segmentations, and protein expression maps from iterative immunolabeling cycles. Data is provided in S3 format with supporting files for visualization.

Use Cases

• Train segmentation models for neuronal morphology based on provided cell segmentations.
• Analyze combinatorial protein barcode patterns based on multiplexed immunostaining data.
• Develop visualization tools for high-resolution microscopy data using precomputed format files.
• Validate model predictions for protein localization using included manual annotations.

Strengths

• Uses expansion microscopy achieving an effective voxel size of ~35 x 35 x 80 nm.
• Includes data from iterative immunolabeling for dozens of molecular targets.
• Provides multiple data types: raw microscopy, segmentations, and precomputed visualizations.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count and dataset size are unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

E11.bio

Collection Method

Generated using PRISM technology combining viral barcoding, expansion microscopy, and iterative immunolabeling.

Time Range

null

Freshness

null

Geography

Mouse hippocampal CA3 region.