Mouse Intestinal Immune Cell Proportions Under Autophagy Deficiency and NEC

Junyu He's dataset contains flow cytometry data from a mouse study investigating the effect of RORγt⁺ cell-specific Atg5 knockout on immune cell populations in necrotizing enterocolitis (NEC). The data includes proportions of total ILC3s, double-negative ILC3 subsets, and CD4⁺RORγt⁺ T cells among CD45⁺ cells, with 6 biological replicates per group. The dataset was last updated on 2026-04 16 and is licensed under CC-BY-4.0.

Use Cases

• Compare immune cell population proportions between autophagy-intact and autophagy-deficient mice based on the described experimental groups.
• Analyze the relationship between autophagy and specific ILC3 subsets (e.g., DN ILC3s) in a disease model based on the detection targets.
• Validate flow cytometry gating strategies for ILC3 and CD4⁺RORγt⁺ T cell identification based on the listed antibody panel.
• Perform statistical comparisons between two independent groups using an unpaired t-test, as described in the experimental design.

Strengths

• Experimental design is clearly described with two genetically defined mouse groups (Atg5fl/fl and RorccreAtg5fl/fl).
• Includes 6 biological replicates per group, providing a basis for statistical analysis.
• Specific detection targets (proportions of total ILC3s, DN ILC3s, and CD4⁺RORγt⁺ T cells) are explicitly listed.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download from the FCS files.
• Row count is unknown, which may limit suitability assessment for certain analyses.
• Data may reflect bias inherent to the specific experimental model (C57BL/6 mice, NEC induction).

Provenance

Source

Junyu He via figshare

Collection Method

Flow cytometry performed on intestinal lamina propria samples from genetically modified mice subjected to experimental NEC induction.

Time Range

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Freshness

Last updated 2026-04-16 02:46:41; freshness should be verified.

Geography

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