Mouse ILC3 ATG5 Expression Under Autophagy Modulation in NEC

Flow cytometry data measuring ATG5 expression in intestinal immune cells from mice with necrotizing enterocolitis. The dataset contains results from 18 mice across three treatment groups, comparing the effects of autophagy inhibition and activation. Author Junyu He published the data on figshare in April 2026 under a CC-BY-4.0 license.

Use Cases

• Compare ATG5 expression levels based on pharmacological autophagy modulation described in the experimental groups.
• Analyze the effect of 3-Methyladenine and Rapamycin treatments on a specific immune cell subset (DN ILC3s) in a disease model.
• Validate statistical findings from the described one-way ANOVA with Tukey’s post-hoc test.
• Benchmark flow cytometry results for ATG5 mean fluorescence intensity in intestinal lamina propria samples.

Strengths

• Includes 6 biological replicates per group, totaling 18 individual mice.
• Experimental design clearly defines three matched groups: DMSO control, 3MA inhibition, and Rapamycin activation.
• Statistical analysis method (one-way ANOVA with Tukey’s test) and significance threshold (P < 0.05) are explicitly stated.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for certain analyses.
• Data is specific to a single mouse strain (C57BL/6) and one disease model, which may limit generalizability.

Provenance

Source

figshare

Collection Method

Experimental data generated via flow cytometry on samples from a standardized mouse model of necrotizing enterocolitis.

Time Range

Experimental timeframe not specified; dataset published in 2026.

Freshness

Last updated 2026-04-16 02:57:21; freshness should be verified.

Geography

Laboratory study; geographic location of the research institution is unknown.