Electronic Supplementary Materials: Freshwater Biomonitoring Data for WFD Compliance

Slovakia is the geographic focus of this dataset, which supports freshwater biomonitoring under the European Water Framework Directive. It contains site characteristics, species read counts from bulk and eDNA samples, and reference metrics for calculating ecological quality ratios across ten stream types. The materials are supplementary to a published article comparing bulk and eDNA metabarcoding performance.

Use Cases

• Comparing species detection between bulk samples and eDNA from water and sediment based on read count tables.
• Calculating multimetric indices for ecological status assessment using provided metric lists and reference values.
• Analyzing sequencing depth and taxonomic richness across different sample types and taxonomic levels.
• Assessing the performance of the ASTERICS system for freshwater biomonitoring in specific stream types.

Strengths

• Contains detailed species-level data with read counts for presence/absence across multiple sample types.
• Provides structured reference values for calculating Ecological Quality Ratios across ten distinct stream types.
• Includes graphical representations of taxonomic detection and sequencing depth, supporting data visualization.

Limitations

• Row counts and specific column names are not provided in any source, limiting precise understanding of data structure.
• The dataset's temporal coverage and exact number of sampling sites are not explicitly stated.
• Contains multiple file formats (XLSX, TIFF) which may require different tools for analysis.

Provenance

Source

Supplementary materials for the article 'Comparative performance of bulk and eDNA metabarcoding data in freshwater biomonitoring under the Water Framework Directive'.

Collection Method

Data gathered via DNA metabarcoding of bulk, water, and sediment samples from freshwater sites.

Freshness

Last updated 2026-03-25 according to datacite and figshare.

Geography

Slovakia