ELONA Assay Results for Tuberculosis Detection in Sputum Samples

ELONA assay results evaluate a single-stranded DNA aptamer for detecting active tuberculosis in sputum samples. The dataset includes results from 68 patient samples, comprising 20 TB patients and 48 control patients. Charlotte Maserumule authored this dataset, which was last updated in April 2026.

Use Cases

• Validate aptamer binding accuracy against the culture positivity reference standard for Mycobacterium tuberculosis.
• Perform ROC curve analysis to determine optimal assay cut-points using sensitivity and specificity metrics.
• Analyze sample-level detection patterns, such as the three cases where only the second sputum sample tested positive.
• Calculate Youden's index to find the best trade-off between sensitivity and specificity from the assay results.

Strengths

• 68 total patient samples provide a basis for preliminary assay evaluation
• Results are benchmarked against a culture positivity reference standard
• Dataset includes a defined control group of 48 patients with latent TB, other symptoms, or healthy status

Limitations

• Small sample size of only 20 confirmed TB patients limits statistical power
• Potential for label noise indicated by two TB-positive donors not detected by the assay
• Limited scope with results from a single aptamer (CSIR 2.11) and one assay type (ELONA)

Provenance

Source

Figshare, authored by Charlotte Maserumule.

Collection Method

Aptamers were screened using an enzyme-linked oligonucleotide assay (ELONA) on sputum samples.

Time Range

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Freshness

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Geography

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