Clostridium Kluyveri Genetic Engineering Data for Plasmid-Based Expression

Ethan M. Agena developed a biparental methylation-conjugation system to enable plasmid delivery in Clostridium kluyveri DSM555, a bacterium used for producing medium-chain carboxylic acids. The dataset, last updated on 2026-03-19, includes results demonstrating heterologous expression of two anaerobic fluorescent reporters, FAST and an evolved FbFP. This work aims to advance metabolic engineering for sustainable chemical production from waste-derived carbon.

Use Cases

• Benchmarking plasmid delivery systems for Clostridium species based on the described methylation-conjugation protocol.
• Analyzing heterologous gene expression efficiency in anaerobic bacteria based on the reported fluorescent reporter data.
• Developing genetic tools for chain-elongating bacteria based on the system designed to bypass host defense mechanisms.

Strengths

• Dataset is associated with a specific, peer-reviewed methodology for bypassing restriction-modification barriers in C. kluyveri.
• Includes demonstration of two distinct heterologous fluorescent reporters (FAST and an evolved FbFP).
• Released under a CC-BY-NC-4.0 license, clarifying reuse terms.

Limitations

• Dataset size is 56.3 KB, indicating a very limited scope of data.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Experimental data from the development and testing of a genetic engineering system.

Freshness

Last updated 2026-03-19 21:05:31