Engineered Cholera Toxin B-Subunit Assemblies via Coiled-Coil Scaffolding

A research dataset from the University of Leeds describes a strategy for assembling proteins into complex structures. The work focuses on the pentameric cholera toxin B-subunit (CTB) and uses appended coiled-coil domains to create tubular supramolecular complexes. The structural analysis was performed using X-ray crystallography.

Use Cases

• Designing hierarchical protein assemblies based on the described coiled-coil scaffolding method.
• Analyzing forced protein-protein interfaces for computational protein design, as highlighted in the description.
• Directing the assembly of oligomeric protein building blocks that present termini on a single face.

Strengths

• Dataset is associated with a specific, peer-reviewed research strategy published on paperswithcode.
• Description details a concrete methodological advance in protein assembly using the cholera toxin B-subunit.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

University of Leeds, authored by Arwen R. Pearson, hosted on paperswithcode.

Collection Method

Research data likely generated from experimental protein engineering and X-ray crystallography.