Engineered Modular Polyketide Synthases Data on Stereocenter Control

Jie Zhang's dataset on figshare, last updated April 22, 2026, contains experimental data on engineered modular polyketide synthases. The 71.9 KB XLSX file likely contains results from mutagenic probing and platform testing to elucidate how enoylreductase domains collaborate with other domains to set stereocenters. This research provides strategies for controlling alkyl substituent orientations in complex polyketides.

Use Cases

• Analyzing the stereoselectivity of enoylreductase (ER) domains based on mutagenic probing data.
• Modeling domain interactions between ER, acyl carrier protein (ACP), and ketosynthase (KS) based on experimental platform results.
• Informing the engineering of stereochemical libraries for polyketide drug leads based on strategies for controlling methyl group orientations.

Strengths

• Data is associated with a specific, peer-reviewed research study elucidating enzymatic mechanisms.
• File is provided in the structured XLSX format, which is widely compatible.
• Dataset has a clear license (CC-BY-NC-4.0) specifying terms of use.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset's 71.9 KB size indicates a very limited scope of experimental results.

Provenance

Source

figshare, authored by Jie Zhang.

Collection Method

Likely contains experimental results from in vitro and in vivo platform testing of engineered synthases.

Time Range

Publication date suggests data is from research conducted prior to April 2026.

Freshness

Last updated 2026-04-22 14:33:42; freshness should be verified.

Geography

Geography is unknown.