Pichia pastoris Engineered for ACV Biosynthesis: Yield and Transcriptomic Data

Shraddha Jogi's dataset documents the reconstruction of the ACV biosynthesis pathway in Pichia pastoris. It includes LC–MS/MS confirmation of ACV production and transcriptomic analysis of the engineered strain. The data was last updated on 2026-05-18.

Use Cases

• Analyze the relationship between genetic modifications and ACV yield based on the described engineering of pcbAB and npgA genes.
• Study metabolic reorganization in engineered yeast based on the transcriptomic analysis mentioned in the description.
• Benchmark heterologous NRP production levels based on the reported ACV yields of ~5-60 ng/mL.
• Investigate the impact of adaptive evolution and precursor supplementation on pathway efficiency based on the described yield improvements.

Strengths

• Includes LC–MS/MS confirmation of the target compound ACV, as stated in the description.
• Reports specific quantitative yield data, including baseline (~5 ng/mL), evolved (~50 ng/mL), and supplemented (~60 ng/mL) levels.
• Contains transcriptomic data to explain metabolic changes in the engineered host.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small in scale at 984.3 KB, indicating a limited scope of data.

Provenance

Source

figshare, author Shraddha Jogi

Collection Method

Experimental data from metabolic engineering and LC–MS/MS analysis.

Freshness

Last updated 2026-05-18 20:05:35; freshness should be verified.