Engineering Pichia pastoris for ACV Biosynthesis: Transcriptomic and Yield Data

Experimental data from a study that successfully reconstructed the ACV biosynthesis pathway in the yeast Pichia pastoris. The dataset includes LC–MS/MS confirmation of ACV production, yield measurements from the starting strain (~5 ng/mL), after adaptive evolution (~50 ng/mL), and with precursor supplementation (~60 ng/mL), and transcriptomic analysis of the engineered strain. The data was published by Shraddha Jogi on figshare in May 2026.

Use Cases

• Benchmarking heterologous expression strategies for non-ribosomal peptide synthetases based on the described engineering of Pichia pastoris.
• Analyzing metabolic adaptations to foreign biosynthetic pathways based on the transcriptomic data mentioned in the description.
• Training models to predict yield improvements from strain engineering steps like adaptive evolution or precursor feeding, based on the reported ACV yield data.

Strengths

• Yield data is quantified with specific measurements (e.g., ~5 ng/mL, ~50 ng/mL, ~60 ng/mL).
• Pathway reconstruction was confirmed using LC–MS/MS analysis.
• Includes transcriptomic analysis to explain metabolic changes in the engineered host.

Limitations

• Dataset size is 48.7 KB, indicating a very limited scope of data.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Experimental study involving genetic engineering, fermentation, LC–MS/MS analysis, and RNA sequencing.

Freshness

Last updated 2026-05-18 20:05:34