Sheep Mycoplasma Pneumonia Epigenetic and Transcriptomic Analysis

High-throughput sequencing techniques (ATAC-Seq and CUT&Tag) were used to analyze epigenetic modifications in lung tissue from healthy and Mycoplasma pneumonia-affected sheep. The study, authored by Kai Huang and last updated in May 2026, integrates transcriptome analysis and GWAS to identify FOXF1 as a candidate resistance gene and validates its role in a cell infection model.

Use Cases

• Identify candidate genes for disease resistance based on integrated epigenetic and transcriptomic data.
• Study the role of FOXF1 in host-pathogen interactions using the described cell infection model.
• Analyze differential epigenetic landscapes between healthy and infected lung tissues.
• Validate genetic markers for selective breeding against Mycoplasma ovipneumoniae.

Strengths

• Integrates multiple high-throughput sequencing methods (ATAC-Seq and CUT&Tag) for epigenetic analysis.
• Includes functional validation through a Mycoplasma ovipneumoniae-infected cell model with gene overexpression and knock-down.
• Released under a permissive CC-BY-4.0 license for reuse.

Limitations

• Row count and column-level documentation are unknown, requiring manual inspection after download.
• The dataset is small (570.4 KB), indicating limited scope or summary-level data.
• Geographic and breed specifics of the sheep samples are not detailed, which may limit generalizability.

Provenance

Source

figshare

Collection Method

High-throughput sequencing (ATAC-Seq, CUT&Tag) and cell culture experiments.

Time Range

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Freshness

Last updated 2026-05 03 14:00:04.

Geography

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