Mouse ILC3 Cell Lipid Metabolism Data from Atg5-Deficient Mice

Flow cytometry data from a 2026 study evaluating lipid metabolism in intestinal immune cells from mice with necrotizing enterocolitis. The dataset contains measurements of lipid accumulation, peroxidation, reactive oxygen species, and fatty acid uptake for two genetically modified mouse groups, with 5 biological replicates per group. It was authored by Junyu He and shared under a CC-BY-4.0 license.

Use Cases

• Compare intracellular lipid accumulation between autophagy-deficient and wild-type ILC3 cells based on BODIPY MFI measurements.
• Assess lipid peroxidation and membrane dynamics in immune cells based on C11-BODIPY MFI data.
• Evaluate levels of intracellular reactive oxygen species based on DCFDA MFI readings.
• Analyze fatty acid uptake or lipid trafficking patterns based on FCL16 MFI values.

Strengths

• Experimental design includes 5 biological replicates per group, providing a basis for statistical analysis.
• Data is focused on a specific immune cell type (ILC3) and a defined genetic knockout (Atg5), enabling precise mechanistic studies.
• File size is 1.9 GB, suggesting the underlying FCS files contain detailed, high-resolution flow cytometry data.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for certain analyses.
• Data is from a single, controlled mouse model study, which may limit generalizability.

Provenance

Source

figshare

Collection Method

Experimental data generated via flow cytometry from genetically modified mouse models.

Time Range

Experimental data likely from a recent study, published in 2026.

Freshness

Last updated 2026-04-16 02:53:13

Geography

Laboratory study; geographic coverage is not specified.