Anti-Toxoplasma gondii IgG ELISA Results for Sun Dried Meat Juice Samples

An evaluation of the ELISA technique for detecting anti-Toxoplasma gondii IgG antibodies in juices from sun dried meat. The dataset likely contains results from 32 experimental beef samples and 42 retail sun dried meat samples, with optical density thresholds determining test viability. The research was conducted by Maria Aparecida Moraes Marciano, focusing on samples from experimentally infected animals and commercial products in northern Brazil.

Use Cases

• Validate ELISA assay protocols for pathogen detection in processed meat based on optical density thresholds mentioned in the description.
• Assess the prevalence of Toxoplasma gondii exposure in cattle and commercial meat products based on sample positivity rates.
• Compare diagnostic sensitivity between experimental and retail meat juice samples based on the described sample groups.
• Investigate risk factors for foodborne toxoplasmosis transmission based on the study's focus on raw/undercooked meat consumption.

Strengths

• Includes results from two distinct sample groups: 32 experimental and 42 retail samples, allowing for comparison.
• Provides specific optical density (OD) threshold (0.116) used to determine test viability, a key methodological detail.
• Reports clear positivity rates: 7 of 9 viable experimental samples and 1 of 6 viable retail samples were positive.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.
• The description indicates a limited potential for meat juice as a biological material in processed meats, as many samples lacked sufficient blood for testing.

Provenance

Source

Maria Aparecida Moraes Marciano

Collection Method

ELISA immunoassay technique applied to meat juice obtained by defrosting sun dried meat.

Geography

Samples include meat from experimentally infected animals and sun dried meats commercialized in shops in northern Brazil.