Evolution-Guided Engineering of Trans-Acyltransferase Polyketide Synthases

ETH Zurich researchers provide data underlying a manuscript on engineering polyketide synthases. The repository contains sequencing data, NMR data, HPLC-MS traces, and bioactivity assay results. Python scripts for data analysis are included.

Use Cases

• Analyze enzyme construct designs based on provided GenBank files.
• Process raw HPLC-MS data using the included Python scripts.
• Evaluate compound bioactivity based on the provided toxicity assay data.
• Validate sequencing results using the provided Sanger and whole plasmid sequencing files.

Strengths

• Data includes multiple raw instrument file types (fastq, mzXML, ab1) for direct analysis.
• Repository provides Python scripts to reproduce figures from the associated manuscript.
• Data covers multiple bacterial sources (Bacillus, Serratia, Gynuella) with some MS-MS data.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

ETH Zurich

Collection Method

Experimental data collected for a research manuscript.