Extended Shine-Dalgarno Motifs in Staphylococcus Aureus

290,100,190 bytes of source data include unprocessed toeprinting films, raw luciferase measurements for reporter assays, polysome profiling data, and raw biofilm analysis data. The dataset supports research into how extended Shine-Dalgarno motifs govern translation initiation in the bacterium Staphylococcus aureus. It contains raw experimental outputs from multiple biochemical and genetic assays.

Use Cases

• Analyzing translation initiation efficiency based on raw luciferase reporter assay measurements.
• Validating ribosome binding sites using unprocessed toeprinting film images.
• Correlating translation rates with biofilm formation using the raw biofilm analysis data.
• Studying ribosome density profiles with the polysome profiling raw data.

Strengths

• Contains 290,100,190 bytes of raw, unprocessed experimental source data, providing a foundation for reproducibility.
• Covers multiple experimental modalities (toeprinting, luciferase assays, polysome profiling, biofilm analysis) for a multi-faceted investigation.
• Released under a permissive CC-BY-4.0 license, facilitating open reuse and sharing.

Limitations

• Column names and a precise row count are not provided, limiting immediate understanding of the data structure.
• The dataset consists of raw, unframed files which likely require significant domain-specific knowledge and preprocessing for analysis.
• The last update date of 2026-03-24 appears to be a future date, indicating a potential metadata error.

Provenance

Source

Maximilian P. Kohl

Collection Method

Experimental data generated from toeprinting, luciferase reporter assays, polysome profiling, and biofilm analysis.

Freshness

Last updated: 2026-03-24 07:02:58 (Note: This is a future date, suggesting a possible metadata error).