OVCAR-3 and Caov-3 Cell Responses to Ferroptosis Inducers and PDT

Experimental data from Marta Overchuk details the differential sensitivity of two ovarian cancer cell lines, OVCAR-3 and Caov-3, to the ferroptosis inducer ML210 and photodynamic therapy (PDT). The dataset includes dose-response curves, cell survival fractions, and measurements of glutathione (GSH) levels, GPX4 protein content, and lipid radical generation using C11 BODIPY fluorescence. It also explores the effects of inhibitors like Lip-1, Fer-1, and the iron chelator DFO on these cytotoxic processes.

Use Cases

• Comparing ferroptosis sensitivity between OVCAR-3 and Caov-3 cell lines based on ML210 dose-response data.
• Analyzing the role of lipid peroxidation in PDT efficacy using oxidized/total C11 BODIPY fluorescence ratios.
• Investigating the protective effects of antioxidants (Lip-1, Fer-1) and iron chelation (DFO) on cell survival.
• Correlating intracellular GSH and GPX4 protein levels with cell viability under different treatment conditions.

Strengths

• Data is structured around specific, reproducible experimental workflows for ML210 and PDT treatments.
• Includes multiple quantitative assays (dose-response, survival fraction, fluorescence ratios) for cross-validation.
• Cross-platform presence on Dataverse suggests the dataset has been shared for verification and reuse.

Limitations

• Critical metadata is missing, including column names, row counts, file formats, and license information.
• The dataset appears to be a collection of figure source data without a unified, machine-readable table structure.
• All platform entries list 'None' for size, rows, and license, indicating poor metadata completeness.

Provenance

Source

Overchuk, Marta; organization listed as 'ODUM Harvested Dataverse'.

Collection Method

Likely generated from laboratory experiments involving cell culture, drug treatment, microscopy, and plate reader assays.

Freshness

Last updated 2026-06-08 (as listed, though this future date is likely a metadata error).