Folate-Liposome Uptake and Binding in Macrophage and Epithelial Cell Lines

Aaron Benjamin published experimental data on June 1, 2026, comparing folate-conjugated liposome uptake in J774A.1 murine macrophages and A549 human epithelial cells using flow cytometry. The dataset includes results from three biological and technical replicates (n=9) for each experiment, with statistical comparisons. Figures detail uptake levels, competitive binding with free folate, and fluorescence retention after cytochalasin D treatment.

Use Cases

• Compare targeted drug carrier uptake between different cell lines based on flow cytometry data.
• Analyze receptor-specific binding through competitive inhibition assays with free folate.
• Investigate the role of phagocytosis in nanoparticle internalization using temperature and cytochalasin D treatments.
• Validate statistical methods for biological replicates in cell-based assays.

Strengths

• Data is derived from three biological and technical replicates, totaling n=9 samples per experiment.
• Statistical analysis is reported, including pairwise T-tests, paired T-tests, and one-way ANOVA.
• Experiments include controls for normalization, such as non-treated cells and 0% folate treatment.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The description is specific to figures; the underlying raw data structure is not detailed.

Provenance

Source

figshare

Collection Method

Experimental data from flow cytometry assays on cell cultures.

Time Range

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Freshness

Last updated 2026-06-01 23:39:05; freshness should be verified.

Geography

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